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This is mostly to get around Seurat complete deprecation of slot leading to the error:

creating vignettes ... ERROR
--- re-building ‘escape.Rmd’ using rmarkdown

Quitting from escape.Rmd:188-200 [unnamed-chunk-10]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
<error/lifecycle_error_deprecated>
Error:
! The `slot` argument of `GetAssayData()` was deprecated in SeuratObject
  5.0.0 and is now defunct.
ℹ Please use the `layer` argument instead.
---
Backtrace:
    ▆
 1. └─escape::escape.matrix(...)
 2.   └─escape:::.cntEval(input.data, assay = "RNA", type = "counts")
 3.     ├─base::suppressWarnings(...)
 4.     │ └─base::withCallingHandlers(...)
 5.     ├─SeuratObject::GetAssayData(obj, assay = assay, slot = type)
 6.     └─SeuratObject:::GetAssayData.Seurat(obj, assay = assay, slot = type)
 7.       └─SeuratObject::.Deprecate(...)
 8.         └─lifecycle::deprecate_stop(...)
 9.           └─lifecycle:::deprecate_stop0(msg)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Error: processing vignette 'escape.Rmd' failed with diagnostics:
The `slot` argument of `GetAssayData()` was deprecated in SeuratObject
5.0.0 and is now defunct.

While at it - I fixed the documentation to be consistent.

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codecov bot commented Jan 4, 2026

Codecov Report

❌ Patch coverage is 90.43478% with 11 lines in your changes missing coverage. Please review.

Files with missing lines Patch % Lines
R/utils.R 88.13% 7 Missing ⚠️
R/geyserEnrichment.R 69.23% 4 Missing ⚠️
Files with missing lines Coverage Δ
R/densityEnrichment.R 98.46% <100.00%> (+0.04%) ⬆️
R/enrichIt.R 92.72% <ø> (ø)
R/enrichItPlot.R 92.64% <100.00%> (ø)
R/getGeneSets.R 69.38% <ø> (ø)
R/gseaEnrichment.R 91.26% <100.00%> (ø)
R/heatmapEnrichment.R 98.00% <100.00%> (ø)
R/pcaEnrichment.R 91.80% <100.00%> (ø)
R/performNormalization.R 75.55% <ø> (ø)
R/performPCA.R 83.33% <ø> (ø)
R/ridgeEnrichment.R 94.73% <100.00%> (ø)
... and 5 more
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@ncborcherding ncborcherding merged commit ade8466 into master Jan 4, 2026
3 checks passed
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2 participants