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+# Readme for the Models Associated with the Paper
+
+Greenstein JL et al., Role of the calcium-independent transient outward current Ito1 in shaping action potential morphology and duration.
+*Circ Res* 2000;87:1026-1033.
+
+## Abstract
+
+The Kv4.3-encoded current I_Kv4.3 has been identified as the major component of the voltage-dependent Ca(2+)-independent transient outward current I_to1 in human and canine ventricular cells. Experimental evidence supports a correlation between I_to1 density and prominence of the phase 1 notch; however, the role of I_to1 in modulating action potential duration (APD) remains unclear. To help resolve this role, Markov state models of the human and canine Kv4.3- and Kv1.4-encoded currents at 35 degrees C are developed on the basis of experimental measurements. A model of canine I_to1 is formulated as the combination of these Kv4.3 and Kv1.4 currents and is incorporated into an existing canine ventricular myocyte model. Simulations demonstrate strong coupling between L-type Ca(2+) current and I_Kv4.3 and predict a bimodal relationship between I_Kv4.3 density and APD whereby perturbations in I_Kv4.3 density may produce either prolongation or shortening of APD, depending on baseline I_to1 current level.
+
+---
+
+The state diagram for this model was shown in figure 1A of the paper.
+Model parameters are available at [http://circres.ahajournals.org/cgi/data/87/11/1026/DC1/1](http://circres.ahajournals.org/cgi/data/87/11/1026/DC1/1)
+
+For the sake of clarity, current traces were displaced in figure 2A.
+Current-voltage relation of this current can also be constructed.
+
+## How to Run the Models
+
+**XPP:** start with the command
+
+```
+xpp ode\hKv4x.ode
+```
+
+Mouse click on **Initialconds**, and then (G)o.
+
+Regarding xpp program, please contact Bard Ermentrout's website [http://www.pitt.edu/~phase/](http://www.pitt.edu/~phase/) which describes how to get and use xpp.
+
+To run a series of voltage-clamp studies, click **Range over**, change to 'vtest_1', and then select voltage protocol from Steps (14), Start (-70) and End (+60).
+This makes traces similar to fig 3A of the paper.
+
+![xpp run](hKv4x.jpg)
+
+## Contact
+
+The model files were submitted by:
+
+Dr. Sheng-Nan Wu, Dr. Ruey J. Sung, Ya-Jean Wang and Jiun-Shian Wu
+National Cheng Kung University Medical College
+Tainan 70101, Taiwan
+e-mail: snwu@mail.ncku.edu.tw
+
+---
+
+2025-05-27 – Standardized to Markdown
\ No newline at end of file
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-<pre>
-This is the readme.txt for the models associated with the paper.
-
-Greenstein JL et al., Role of the calcium-independent transient outward 
-current Ito1 in shaping action potential morphology and duration.
-Circ Res 2000;87:1026-1033.
-
-Abstract:
-The Kv4.3-encoded current I_Kv4.3 has been identified as the major
-component of the voltage-dependent Ca(2+)-independent transient
-outward current I_to1 in human and canine ventricular
-cells. Experimental evidence supports a correlation between I_to1
-density and prominence of the phase 1 notch; however, the role of
-I_to1 in modulating action potential duration (APD) remains
-unclear. To help resolve this role, Markov state models of the human
-and canine Kv4.3- and Kv1.4-encoded currents at 35 degrees C are
-developed on the basis of experimental measurements. A model of canine
-I_to1 is formulated as the combination of these Kv4.3 and Kv1.4
-currents and is incorporated into an existing canine ventricular
-myocyte model. Simulations demonstrate strong coupling between L-type
-Ca(2+) current and I_Kv4.3 and predict a bimodal relationship
-between I_Kv4.3 density and APD whereby perturbations in I_Kv4.3
-density may produce either prolongation or shortening of APD,
-depending on baseline I_to1 current level.
--------------------------------------
-
-The state diagram for this model was shown in figure 1A of the paper.
-Model parameters are available at<a href="http://circres.ahajournals.org/cgi/data/87/11/1026/DC1/1">.
-http://circres.ahajournals.org/cgi/data/87/11/1026/DC1/1</a>
-For the sake of clarity, current traces were displaced in figure 2A.
-Current-voltage relation of this current can also be constructed.
-
-To run the models:
-XPP: start with the command
-
-xpp ode\hKv4x.ode
-
-Mouse click on Initialconds, and then (G)o.
-
-Regarding xpp program, please contact with 
-Bard Ermentrout's website <a href="http://www.pitt.edu/~phase/">http://www.pitt.edu/~phase/</a>
-describes how to get and use xpp.
-
-To run a series of voltage-clamp studies, click Range over, 
-change to 'vtest_1', 
-and then select voltage protocol from Steps (14), Start (-70) and End (+60).
-This makes traces:
-
-<img src="hKv4x.jpg" alt="xpp run">
-
-similar to fig 3A of the paper.
-
-The model files were submitted by:
-
-Dr. Sheng-Nan Wu, Dr. Ruey J. Sung, Ya-Jean Wang and Jiun-Shian Wu
-National Cheng Kung University Medical College
-Tainan 70101, Taiwan
-e-mail: snwu@mail.ncku.edu.tw
-</pre>